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c jun si rna  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology c jun si rna
    C Jun Si Rna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+jun+si+rna/pm37175814-239-47-51?v=Santa+Cruz+Biotechnology
    Average 93 stars, based on 97 article reviews
    c jun si rna - by Bioz Stars, 2026-08
    93/100 stars

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    Cells were transfected with either <t>PKR</t> <t>siRNA</t> or control siRNA, then PKR mRNA was quantified by real-time RT-PCR; PKR was knocked down (A). Cells were transfected with PKR-expression plasmid (pPKR) or control plasmid (pOS8), then subjected to real-time RT-PCR; PKR mRNA was upregulated by pPKR (B). Mean ± SEM of six replicates. **p<0.01. The amount of HCV core protein in JFH1 and H77s cells were measured by ELISA. HCV core protein was stably expressed in both cell types at least three days after transfection with the <t>HCV-RNA</t> (C). The amount of HCV mRNA in JFH1 and H77s cells was measured by real-time RT-PCR. HCV RNA was expressed in both cell types at least three days after transfection with the HCV-RNA (D). Mean ± SEM of four replicates. PKR and phosphorylated PKR protein expression evaluated by Western blotting; results confirmed PKR mRNA data (E).
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    Cells were transfected with either PKR siRNA or control siRNA, then PKR mRNA was quantified by real-time RT-PCR; PKR was knocked down (A). Cells were transfected with PKR-expression plasmid (pPKR) or control plasmid (pOS8), then subjected to real-time RT-PCR; PKR mRNA was upregulated by pPKR (B). Mean ± SEM of six replicates. **p<0.01. The amount of HCV core protein in JFH1 and H77s cells were measured by ELISA. HCV core protein was stably expressed in both cell types at least three days after transfection with the HCV-RNA (C). The amount of HCV mRNA in JFH1 and H77s cells was measured by real-time RT-PCR. HCV RNA was expressed in both cell types at least three days after transfection with the HCV-RNA (D). Mean ± SEM of four replicates. PKR and phosphorylated PKR protein expression evaluated by Western blotting; results confirmed PKR mRNA data (E).

    Journal: PLoS ONE

    Article Title: Protein Kinase R Modulates c-Fos and c-Jun Signaling to Promote Proliferation of Hepatocellular Carcinoma with Hepatitis C Virus Infection

    doi: 10.1371/journal.pone.0067750

    Figure Lengend Snippet: Cells were transfected with either PKR siRNA or control siRNA, then PKR mRNA was quantified by real-time RT-PCR; PKR was knocked down (A). Cells were transfected with PKR-expression plasmid (pPKR) or control plasmid (pOS8), then subjected to real-time RT-PCR; PKR mRNA was upregulated by pPKR (B). Mean ± SEM of six replicates. **p<0.01. The amount of HCV core protein in JFH1 and H77s cells were measured by ELISA. HCV core protein was stably expressed in both cell types at least three days after transfection with the HCV-RNA (C). The amount of HCV mRNA in JFH1 and H77s cells was measured by real-time RT-PCR. HCV RNA was expressed in both cell types at least three days after transfection with the HCV-RNA (D). Mean ± SEM of four replicates. PKR and phosphorylated PKR protein expression evaluated by Western blotting; results confirmed PKR mRNA data (E).

    Article Snippet: Control siRNA was obtained from Cosmo Bio (Tokyo, Japan); c-Fos siRNA and c-Jun si-RNA were obtained from Thermo Fisher Scientific.

    Techniques: Transfection, Quantitative RT-PCR, Expressing, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Stable Transfection, Western Blot

    RNA from HCC specimens of 34 patients, 17 HCC specimens with HCV infection (HCC positive HCC), and 17 HCC without HCV infection (HCC negative HCC). Each group was divided into two subsections by the median PKR mRNA values: Low PKR and High PKR. c-Jun mRNA (A) and c-Fos mRNA (B) were measured. c-Fos mRNA significantly correlated with c-Jun mRNA (r = 0.816, P<0.001) (C). The four human HCC specimens having the highest (High PKR) and the four having the lowest (Low PKR) PKR protein expression were analyzed by Western blotting. c-Jun and c-Fos in the High PKR group were activated more than in the Low PKR group (D).

    Journal: PLoS ONE

    Article Title: Protein Kinase R Modulates c-Fos and c-Jun Signaling to Promote Proliferation of Hepatocellular Carcinoma with Hepatitis C Virus Infection

    doi: 10.1371/journal.pone.0067750

    Figure Lengend Snippet: RNA from HCC specimens of 34 patients, 17 HCC specimens with HCV infection (HCC positive HCC), and 17 HCC without HCV infection (HCC negative HCC). Each group was divided into two subsections by the median PKR mRNA values: Low PKR and High PKR. c-Jun mRNA (A) and c-Fos mRNA (B) were measured. c-Fos mRNA significantly correlated with c-Jun mRNA (r = 0.816, P<0.001) (C). The four human HCC specimens having the highest (High PKR) and the four having the lowest (Low PKR) PKR protein expression were analyzed by Western blotting. c-Jun and c-Fos in the High PKR group were activated more than in the Low PKR group (D).

    Article Snippet: Control siRNA was obtained from Cosmo Bio (Tokyo, Japan); c-Fos siRNA and c-Jun si-RNA were obtained from Thermo Fisher Scientific.

    Techniques: Infection, Expressing, Western Blot